B06 Colorimetric Glucose Determination with Glucose Oxidase

Description
An orange color is generated as the product of two enzyme reactions. First glucose oxidase oxidizes glucose and forms hydrogen peroxide. The hydrogen peroxide oxidation of o-dianisidine is then catalyzed by horseradish peroxidase to form an orange compound. Three different concentrations of glucose are shown.

Equation
glucose + H₂O + O₂ (glucose oxidase cat.) --> gluconate + H₂O₂

H₂O₂+ o-dianisidine (Peroxidase cat. )--> oxidized o-dianisidine

Reference
Sigma Chemical Company Procedure No. 510, 1989 Catalog.