B09 Kinetics of Glucose Oxidation Determined by a Specific Ion Electrode

Description
The fluoride ion specific electrode is used to detect products of the glucose oxidase catalyzed oxidation of glucose. Horseradish peroxidase catalyzes the oxidation of o-fluorophenol and produces free fluoride ion which is detected by the ion specific electrode. In order to illustrate how membrane electrodes work, the enzymes are placed in a tube separated by dialysis tubing from the sample. Data was recorded as a function of time and the chart recordings are displayed for two enzyme concentrations, two glucose concentrations, and two fluorophenol concentrations. The data may be used for kinetic rate law discussions.

Equation
glucose + H₂O + O₂ (glucose oxidase cat. ) --> gluconate + H₂O₂

H₂O₂ + o-fluorophenol (Peroxidase cat. ) --> F^-+ oxidized o-?phenol

Reference
I. W. Siddiqi, Clinical Chemistry,Vol.28,No. 9, (1982), p.1962.

See experiments B06 and B08 also.